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Comprehensive two-dimensional liquid chromatography: ion chromatography × reversed-phase liquid chromatography for separation of low-molar-mass organic acids
journal contribution
posted on 2010-10-22, 00:00 authored by Stella S Brudin, Robert ShellieRobert Shellie, Paul R Haddad, Peter J SchoenmakersIn the work presented here a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Ion chromatography is chosen for the first-dimension separation and reversed-phase liquid chromatography is chosen for the second-dimension separation mode. The coupling of these modes is made possible by neutralising the first-dimension effluent, containing KOH, prior to transfer to the second-dimension reversed-phase column. A test mixture of 24 low-molar-mass organic acids is used for optimisation of the system. Three food and beverage samples were analysed in order to evaluate the developed methodology, the resulting two-dimensional separation is near-orthogonal, the set-up is simple and all instrumental components are available commercially. The method proved to be robust and suitable for the analysis of wine, orange juice and yogurt.
History
Journal
Journal of chromatography AVolume
1217Issue
43Pagination
6742 - 6746Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
eISSN
1873-3778Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2010, Elsevier B.V.Usage metrics
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No categories selectedKeywords
BeveragesCarboxylic AcidsChromatography, Ion ExchangeChromatography, Reverse-PhaseMolecular WeightRegression AnalysisReproducibility of ResultsSensitivity and SpecificityWineYogurtScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryIon chromatographyReversed phaseOrganic acidsMultidimensional liquid chromatographyOrthogonal separationCOLUMNPERFORMANCESYSTEM
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