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Concepts and preliminary observations on the triple-dimensional analysis of complex volatile samples by using GC×GC-TOFMS
journal contribution
posted on 2001-01-01, 00:00 authored by Robert ShellieRobert Shellie, P Marriott, P MorrisonThe high-resolution two-dimensional comprehensive gas chromatography (GC×GC) separation of a complex sample of an essential oil is reported, with tentative identification of selected separated components provided by time-off-light mass spectrometry (TOFMS). The GC×GC technique allows orthogonal separation mechanisms on the two columns to achieve separation of components that otherwise are unresolved on a single column, as is demonstrated for the pairs of components borneol and terpinen-4-ol, and cis-caryophyllene and β-farnesene. Peak compression and a short second column used in GC×GC lead to generation of fast second-dimension GC peaks and higher detection sensitivity, by about 25 times, as compared to conventional GC elution. This allows many more compounds to be recognized when using the GC×GC approach. Additionally, rapid mass spectral methods are required if accurate data and reliable searchable spectra are to be obtained for the fast peaks; this is achieved with TOFMS. This leads to a three-dimensional analytical technique. Application of the technique to the complex essential oil sample containing a range of chemical compound classes shows that superior separation and more accurate peak assignment results. Once peaks are identified within the two-dimensional separation space, it is conceivable that mass spectrometry might no longer be required for the routine analysis of such samples, instead relying on the precision of flame ionization detection to give quantitative analysis; however, the support of mass spectral characterization will be invaluable in validating the GC×GC approach.
History
Journal
Analytical chemistryVolume
73Issue
6Pagination
1336 - 1344Publisher
American Chemical SocietyLocation
Washington, D.C.Publisher DOI
ISSN
0003-2700Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2001, American Chemical SocietyUsage metrics
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