stambas-consequencesof-2005.pdf (357.87 kB)
Consequences of immunodominant epitope deletion for minor influenza virus-specific CD8+-T-cell responses
journal contribution
posted on 2005-01-01, 00:00 authored by S Andreansky, John StambasJohn Stambas, P Thomas, W Xie, R Webby, P DohertyThe extent to which CD8+ T cells specific for other antigens expand to compensate for the mutational loss of the prominent DbNP366 and DbPA224 epitopes has been investigated using H1N1 and H3N2 influenza A viruses modified by reverse genetics. Significantly increased numbers of CD8+ KbPB1703+ , CD8+ KbNS2114+, and CD8+ DbPB1-F262+ T cells were found in the spleen and in the inflammatory population recovered by bronchoalveolar lavage from mice that were first given the -NP-PA H1N1 virus intraperitoneally and then challenged intranasally with the homologous H3N2 virus. The effect was less consistent when this prime-boost protocol was reversed. Also, though the quality of the response measured by cytokine staining showed some evidence of modification when these minor CD8+-T-cell populations were forced to play a more prominent part, the effects were relatively small and no consistent pattern emerged. The magnitude of the enhanced clonal expansion following secondary challenge suggested that the prime-boost with the -NP-PA viruses gave a response overall that was little different in magnitude from that following comparable exposure to the unmanipulated viruses. This was indeed shown to be the case when the total response was measured by ELISPOT analysis with virus-infected cells as stimulators. More surprisingly, the same effect was seen following primary challenge, though individual analysis of the CD8+ KbPB1703+ , CD8+ KbNS2114+, and CD8+ DbPB1-F262+ sets gave no indication of compensatory expansion. A possible explanation is that novel, as yet undetected epitopes emerge following primary exposure to the -NP-PA deletion viruses. These findings have implications for both natural infections and vaccines.
History
Journal
Journal of VirologyVolume
79Issue
7Pagination
4329 - 4339Publisher
American Society for MicrobiologyLocation
Washington, D.C.Publisher DOI
ISSN
0022-538XeISSN
1098-5514Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2005, American Society for MicrobiologyUsage metrics
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