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Copper and zinc mediated oligomerisation of Aß peptides
journal contribution
posted on 2006-06-01, 00:00 authored by F Ali, F Separovic, Colin BarrowColin Barrow, S Yao, K BarnhamThe accumulation of senile plaques composed primarily of aggregated amyloid β-peptide (Aβ), is the major characteristic of Alzheimer’s disease. Many studies correlate plaque accumulation and the presence of metal ions, particularly copper and zinc. The metal binding sites of the amyloid Aβ peptide of Alzheimer’s disease are located in the N-terminal region of the full-length peptide. In this work, the interactions with metals of a model peptide comprising the first 16 amino acid residues of the amyloid Aβ peptide, Aβ(1–16), were studied. The effect of Cu2+ and Zn2+ binding to Aβ(1–16) on peptide structure and oligomerisation are reported. The results of ESI-MS, gel filtration chromatography and NMR spectroscopy demonstrated formation of oligomeric complexes of the peptide in the presence of the metal ions and revealed the stoichiometry of Cu2+ and Zn2+ binding to Aβ(1–16), with Cu2+ showing a higher affinity for binding the peptide than Zn2+.
History
Journal
International journal of peptide research and therapeuticsVolume
12Issue
2Pagination
153 - 164Publisher
Springer NetherlandsLocation
Amsterdam, NetherlandsPublisher DOI
ISSN
1573-3149eISSN
1573-3904Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2006, Springer Science+Business MediaUsage metrics
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