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Counter-pressure-assisted ITP with electrokinetic injection under field-amplified conditions for bacterial analysis

Version 2 2024-06-04, 12:13
Version 1 2016-07-15, 17:21
journal contribution
posted on 2024-06-04, 12:13 authored by SC Phung, YH Nai, M Macka, SM Powell, Rosanne GuijtRosanne Guijt, MC Breadmore
Counter-pressure was used to extend the duration of field-amplified sample injection in isotachophoresis (FASI-ITP) in order to improve the detection of bacterial cells. Using 0.51-μm negatively charged encapsulated fluorescent beads as a model, the counter-pressure, injection and separation voltages, and times were optimized. Using 6-min 8,963-Pa counter-pressure FASI-ITP injections at −12 kV followed by mobilization of the ITP band with continued injection at −6 kV, the limit of detection (LOD) for Escherichia coli was improved to 78 cells/mL, a factor of 4 when compared with FASI-ITP without counter-pressure.

History

Journal

Analytical and bioanalytical chemistry

Volume

407

Pagination

6995-7002

Location

Berlin, Germany

ISSN

1618-2642

eISSN

1618-2650

Language

eng

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2015, Springer

Publisher

Springer