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Counter-pressure-assisted ITP with electrokinetic injection under field-amplified conditions for bacterial analysis
journal contribution
posted on 2015-09-01, 00:00 authored by S C Phung, Ryan Nai, M Macka, S M Powell, Rosanne GuijtRosanne Guijt, M C BreadmoreCounter-pressure was used to extend the duration of field-amplified sample injection in isotachophoresis (FASI-ITP) in order to improve the detection of bacterial cells. Using 0.51-μm negatively charged encapsulated fluorescent beads as a model, the counter-pressure, injection and separation voltages, and times were optimized. Using 6-min 8,963-Pa counter-pressure FASI-ITP injections at −12 kV followed by mobilization of the ITP band with continued injection at −6 kV, the limit of detection (LOD) for Escherichia coli was improved to 78 cells/mL, a factor of 4 when compared with FASI-ITP without counter-pressure.
History
Journal
Analytical and bioanalytical chemistryVolume
407Pagination
6995 - 7002Publisher
SpringerLocation
Berlin, GermanyPublisher DOI
ISSN
1618-2642eISSN
1618-2650Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, SpringerUsage metrics
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No categories selectedKeywords
capillary electrophoresis\/electrophoresiscell systems\/single cell analysiscounter-pressurecapillary isotachophoresismicrobialseletrokinetic injectionsfield amplifiedScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryElectrokinetic injectionsCAPILLARY-ZONE-ELECTROPHORESISISOTACHOPHORETIC STACKINGHYDRODYNAMIC INJECTIONANIONS
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