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Critical assessment of various techniques for the extraction of carotenoids and co-enzyme Q10 from the Thraustochytrid strain ONC-T18

journal contribution
posted on 2006-12-27, 00:00 authored by R Armenta, A Burja, H Radianingtyas, Colin BarrowColin Barrow
A variety of techniques for extracting carotenoids from the marine Thraustochytrium sp. ONC-T18 was compared. Specifically, the organic solvents acetone, ethyl acetate, and petroleum ether were tested, along with direct and indirect ultrasonic assisted extraction (probe vs bath) methods. Techniques that used petroleum ether/acetone/water (15:75:10, v/v/v) with 3 h of agitation, or 5 min in an ultrasonic bath, produced the highest extraction yields of total carotenoids (29−30.5 μg g-1). Concentrations up to 11.5 μg g-1 of canthaxanthin and 17.5 μg g-1 of β-carotene were detected in extracts stored for 6 weeks. Astaxanthin and echinenone were also detected as minor compounds. Extracts with and without antioxidants showed similar carotenoid concentration profiles. However, total carotenoid concentrations were approximately 8% higher when antioxidants were used. Finally, an easy-to-perform and inexpensive method to detect co-enzymes in ONC-T18 was also developed using silica gel TLC plates. Five percent methanol in toluene as a mobile phase consistently eluted co-enzyme Q10 standards and could separate the co-enzyme fractions present in ONC-T18.

History

Journal

Journal of agricultural and food chemistry

Volume

54

Issue

26

Pagination

9752 - 9758

Publisher

American Chemical Society

Location

Columbus, Ohio

ISSN

0021-8561

eISSN

1520-5118

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2006, American Chemical Society