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Dengue virus infection changes Aedes aegypti oviposition olfactory preferences

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journal contribution
posted on 2018-09-04, 00:00 authored by Julie Gaburro, Prasad N Paradkar, Melissa Klein, Asim BhattiAsim Bhatti, Saeid NahavandiSaeid Nahavandi, Jean-Bernard Duchemin
Aedes aegypti mosquitoes, main vectors for numerous flaviviruses, have olfactory preferences and are capable of olfactory learning especially when seeking their required environmental conditions to lay their eggs. In this study, we showed that semiochemical conditions during Aedes aegypti larval rearing affected future female choice for oviposition: water-reared mosquitoes preferred to lay eggs in water or p-cresol containers, while skatole reared mosquitoes preferred skatole sites. Using two independent behavioural assays, we showed that this skatole preference was lost in mosquitoes infected with dengue virus. Viral RNA was extracted from infected female mosquito heads, and an increase of virus load was detected from 3 to 10 days post infection, indicating replication in the insect head and possibly in the central nervous system. Expression of selected genes, potentially implied in olfactory learning processes, were also altered during dengue infection. Based on these results, we hypothesise that dengue virus infection alters gene expression in the mosquito's head and is associated with a loss of olfactory preferences, possibly modifying oviposition site choice of female mosquitoes.

History

Journal

Scientific reports

Volume

8

Article number

13179

Pagination

1 - 11

Publisher

Nature Publishing Group

Location

London, Eng.

eISSN

2045-2322

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2018, The Authors