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Designing melittin-graphene hybrid complexes for enhanced antibacterial activity
journal contribution
posted on 2019-05-09, 00:00 authored by Xuemei Lu, Jiaojiao Liu, Lu Gou, Jingliang LiJingliang Li, Bing Yuan, Kai Yang, Yuqiang MaAntimicrobial peptides (AMPs) promise a fundamental solution to the devastating threat of drug-resistant bacteria. However, drawbacks of AMPs (e.g., poor cell membrane penetration efficiency) seriously block their clinical use. In this work, rational design of a hybrid complex of melittin (as a representative AMP) and graphene or graphene oxide (Gra or GO) nanosheets for enhanced antibacterial ability is achieved, via combining in-silico prediction and in-tube test. In comparison to pristine melittin, the specifically designed AMP-Gra (/GO) complex exhibits remarkable efficiency in transmembrane perforation with an over tenfold decrease in the threshold working concentration of peptide; moreover, it has an up to 20-fold enhancement in antibacterial activity against both Gram-negative and Gram-positive bacteria. Such improvement is ascribed to the synergetic insertion of nanosheets and melittin due to similarity in antibacterial mechanism between them and is further regulated by the structural factors of the complex, including the intersheet spacing and surface functionalization of the Gra/GO sheets, etc. These results provide practical guidelines to engineer AMPs with nanotechnology for improved antimicrobial performances, especially based on targeted functionalization of the Gra/GO nanosheets.
History
Journal
Advanced healthcare materialsVolume
8Issue
9Article number
1801521Pagination
1 - 10Publisher
John Wiley & SonsLocation
Chichester, Eng.Publisher DOI
eISSN
2192-2659Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2019, WILEY‐VCH Verlag GmbH & Co. KGaA, WeinheimUsage metrics
Keywords
antimicrobial peptidesgraphene (oxide)hybrid complexeslipid bilayersmelittinScience & TechnologyTechnologyEngineering, BiomedicalNanoscience & NanotechnologyMaterials Science, BiomaterialsEngineeringScience & Technology - Other TopicsMaterials ScienceQUATERNARY AMMONIUMMOLECULAR-DYNAMICSMEMBRANESBACTERIANANOPARTICLESEXTRACTIONHYDROGELSINSIGHTSFABRICSFunctional MaterialsNanomaterialsComposite and Hybrid Materials
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