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Detailed Transcription Map of Aleutian Mink Disease Parvovirus.

Version 2 2024-06-04, 06:34
Version 1 2017-05-17, 14:16
journal contribution
posted on 2024-06-04, 06:34 authored by Soren AlexandersenSoren Alexandersen, ME Bloom, S Perryman
We studied the transcription program of Aleutian mink disease parvovirus (ADV) by using a combination of cDNA cloning and sequencing, primer extension, and Northern (RNA) blot hybridization with splice-specific oligonucleotides. The 4.8-kilobase ADV genome was transcribed in the rightward direction, yielding plus-sense polyadenylated transcripts of 4.3 (R1 RNA), 2.8 (R2), 2.8 (R3), 1.1 (RX), and 0.85 (R2') kilobases. Each RNA transcript had potential translation initiation sites within open reading frames, suggesting protein translation, and a scheme encompassing ADV structural and nonstructural proteins is proposed. Each of the five RNA transcripts had a characteristic set of splices and originated from a promoter at nucleotide 152 (map unit 3 [R1, R2, R2', and RX]) or at nucleotide 1729 (map unit 36 [R3]). The transcripts terminated with a poly(A) tail at one of two positions: either at map unit 53 (R2' and RX) or at map unit 92 (R1, R2, and R3). Similarities with and differences from the transcription maps of other parvoviruses are discussed, and possible roles of the unique features found in ADV transcription are related to the special pathogenic features of this virus.

History

Journal

Journal of Virology

Volume

62

Pagination

3684-3694

Location

United States

ISSN

0022-538X

Language

eng

Publication classification

CN.1 Other journal article

Issue

10

Publisher

American Society for Microbiology