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Detection for folding of the thrombin binding aptamer using label-free electrochemical methods

journal contribution
posted on 2023-02-05, 22:45 authored by M Cho, Y Kim, S Y Han, K Min, Md Aminur Rahman, Y B Shim, C Ban
The folding of aptamer immobilized on an Au electrode was successfully detected using label-free electrochemical methods. A thrombin binding DNA aptamer was used as a model system in the presence of various monovalent cations. Impedance spectra showed that the extent to which monovalent cations assist in folding of aptamer is ordered as K+ NH4+ > Na+ > Cs+. Our XPS analysis also showed that K+ and NH4+ caused a conformational change of the aptamer in which it forms a stable complex with these monovalent ions. Impedance results for the interaction between aptamer and thrombin indicated that thrombin interacts more with folded aptamer than with unfolded aptamer. The EQCM technique provided a quantitative analysis of these results. In particular, the present impedance results showed that thrombin participates a folding of aptamer to some extent, and XPS analysis confirmed that thrombin stabilizes and induces the folding of aptamer.

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Journal

Journal of Biochemistry and Molecular Biology

Volume

41

Pagination

126 - 131

ISSN

1225-8687

eISSN

0219-1024

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