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Detection of Dichelobacter nodosus using species-specific oligonucleotides as PCR primers
journal contributionposted on 1993-05-01, 00:00 authored by Sharon La FontaineSharon La Fontaine, J R Egerton, J I Rood
Dichelobacter nodosus is an essential causative agent of ovine footrot, a disease of major economic significance. Four oligonucleotides complementary to variable regions of the 16S rRNA of D. nodosus were identified, synthesized and tested for their specificity and sensitivity as probes for the detection of D. nodosus. In hybridization reactions using total RNA as the target nucleic acid, three probes were found to be both sensitive and species-specific. When these probes were used as primers in PCR reactions, on both purified D. nodosus DNA and whole cells, the sensitivity of detection was increased by several orders of magnitude. Using PCR, it was possible to detect the presence of D. nodosus by direct examination of lesion material from footrot infected sheep.
Pagination101 - 117
LocationAmsterdam, The Netherlands
Publication classificationC1.1 Refereed article in a scholarly journal
Copyright notice1993, Elsevier B.V.
AnimalsBacteroidesBacteroides InfectionsBase SequenceBlotting, NorthernBlotting, SouthernDNA, BacterialDNA, RibosomalFoot RotMaleMolecular Sequence DataOligonucleotide ProbesPolymerase Chain ReactionRNA, BacterialRNA, Ribosomal, 16SSensitivity and SpecificitySheepSheep DiseasesScience & TechnologyLife Sciences & BiomedicineMicrobiologyVeterinary Sciences16S RIBOSOMAL-RNAPOLYMERASE CHAIN-REACTIONBACTEROIDES-NODOSUSVARIABLE REGIONSNUCLEIC-ACIDSDNA PROBESOLIGODEOXYNUCLEOTIDE PROBESKINGELLA-INDOLOGENESTREPONEMA-PALLIDUMIDENTIFICATIONMicrobiology