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Detection of Entamoeba histolytica antigens in stool in amebiasis.

Version 2 2024-06-13, 10:46
Version 1 2017-07-27, 11:38
journal contribution
posted on 2024-06-13, 10:46 authored by VK Vinayak, P Kumar, V Punj, N Vashist, JR Kanwar, DK Bhasin, K Singh
BACKGROUND: Stool microscopy, the conventional method of diagnosing intestinal amebiasis, fails to detect Entamoeba histolytica in more than 30-40% of clinically suspected cases. Demonstration of parasite products in clinical specimens has been suggested as an alternative. However, the usefulness of demonstrating amebic antigen in the stools of clinical cases needs to be assessed. METHODS: A double-antibody sandwich enzyme linked immunosorbent assay (ELISA) using anti-trophozoite antibodies to capture E histolytica specific coproantigen(s) was carried out on stools obtained from 31 patients with microscopically confirmed non-dysenteric amebic colitis, 18 patients with intestinal parasites other than E histolytica and 41 apparently healthy subjects. RESULTS: The assay detected E histolytica specific coproantigen(s) in stools of 23 (74.2%) of 31 subjects with non-dysenteric amebic colitis, none of 18 with other parasitic infections and 1 (2.4%) of 41 apparently healthy subjects. CONCLUSION: Our results provide evidence for the presence of E histolytica specific coproantigen(s) in stool eluates from patients with amebic infection; this finding can be exploited for confirming ongoing amebic infection. However, the sensitivity of the assay needs to be improved by the use of relevant monospecific/monoclonal antibodies.

History

Journal

Indian Journal of Gastroenterology

Volume

12

Pagination

77-79

Location

India

ISSN

0254-8860

Language

eng

Publication classification

CN.1 Other journal article

Issue

3

Publisher

Indian Society of Gastroenterology

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