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Detection of biogenic amines in pet food ingredients by RP-HPLC with automated dansyl chloride derivatization

journal contribution
posted on 2018-12-01, 00:00 authored by Jessica LeareyJessica Learey, Shona Crawford-Clark, Benjamin J Bowen, Colin BarrowColin Barrow, Jacqui AdcockJacqui Adcock
The detection of biogenic amines is of significant interest to the food industry, as they can be used as indicators of food spoilage and they are potentially toxic. Because of their importance, there is a need for automated methods suitable for industry use that can detect a wide range of biogenic amines at sufficient levels for food analysis. In this work, optimized conditions for the automated determination of biogenic amines (histamine, putrescine, cadaverine, spermine, spermidine, tyramine, and tryptamine) derivatized with dansyl chloride are presented. Limits of detection below 0.2 ppm were achieved for seven biogenic amines and percentage recoveries were between 80 and 109% for the seven analytes spiked into meat meal samples. The method is simple and compared well to an existing method for the detection of biogenic amines in pet food ingredients.

History

Journal

Journal of separation science

Volume

41

Issue

24

Pagination

4430 - 4436

Publisher

Wiley

Location

Weinheim, Germany

ISSN

1615-9306

eISSN

1615-9314

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2018, WILEY-VCH Verlag GmbH