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Detection of Endogenous Phosphatidylinositol 4,5–bisphosphate in Phytophthora cinnamomi

Version 2 2024-06-03, 20:11
Version 1 2018-10-25, 11:43
journal contribution
posted on 2024-06-03, 20:11 authored by JM Howard, PL Beech, Damien CallahanDamien Callahan
Plant diseases caused by Phytophthora species are serious threats to agriculture and the natural environment. Genome sequencing has revealed the lack of a gene for canonical phospholipase C (PLC), an enzyme that was hitherto thought to be ubiquitous in eukaryotes. PLC acts in the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2 ), a membrane-bound phospholipid critical for signal initiation in many cellular processes. Previous studies have not provided evidence of endogenous PtdIns-4,5-P2 in Phytophthora and, in the absence of canonical PLC, argued for redundancy or loss in the PLC pathway in Phytophthora. Using liquid chromatography mass spectrometry, we have detected endogenous PtdIns-4,5-P2 in Phytophthora cinnamomi. This is the first identification of the phospholipid in the genus, and is significant because it indicates that the signal transduction pathway of the PLC product, inositol 1,4,5-trisphosphate (IP3 ), may have been retained in Phytophthora incorporating an as-yet unidentified homolog or analog of PLC.

History

Journal

Lipids

Volume

53

Pagination

835-839

Location

United States

ISSN

0024-4201

eISSN

1558-9307

Language

English

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2018, AOCS

Issue

8

Publisher

WILEY