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Detection of pyrrolizidine alkaloids using flow analysis with both acidic potassium permanganate and tris(2,2’-bipyridyl)ruthenium(II) chemiluminescence
journal contribution
posted on 2005-06-01, 00:00 authored by B Gorman, Neil BarnettNeil Barnett, Richard BosFor the first time, analytically useful chemiluminescence was elicited from the reactions of the pyrrolizidine alkaloids. Heliotrine, retronecine, supinine, monocrotaline and echinatine N-oxide yielded chemiluminescence upon reaction with tris(2,2′-bipyridyl)ruthenium(II) whilst lasiocarpine, its N-oxide and supinine elicited light upon reaction with acidic potassium permanganate. Detection limits for heliotrine were 1.25 × 10−7 M and 9 × 10−9 M for tris(2,2′-bipyridyl)ruthenium(III) perchlorate with flow injection analysis (FIA) and the silica-immobilised reagent (4-[4-(dichloromethylsilanyl)-butyl]-4′-methyl-2,2′-bipyridine)bis(2,2′-bipyridyl)ruthenium(II) with sequential injection analysis (SIA), respectively. Lasiocarpine was detectable at 1.4 × 10−7 M using acidic potassium permanganate with FIA. Additionally, the silica-immobilised reagent was optimised with respect to the oxidant (ammonium ceric nitrate) concentration and the aspiration times which afforded a detection limit for codeine of 5 × 10−10 M using SIA.
History
Journal
Analytica chimica actaVolume
541Issue
1-2Pagination
119 - 124Publisher
Elsevier BVLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0003-2670eISSN
1873-4324Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2004, Elsevier B.V.Usage metrics
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pyrrolizidine alkaloidschemiluminescenceimmobilised tris(22′-bipyridyl)ruthenium(II)sequential injectionScience & TechnologyPhysical SciencesChemistry, AnalyticalChemistryimmobilised tris(2,2 '-bipyridyl)ruthenium(II)CHROMATOGRAPHY MASS-SPECTROMETRYPERFORMANCE LIQUID-CHROMATOGRAPHYCAPILLARY GAS-CHROMATOGRAPHYSOLID-PHASE EXTRACTIONREAGENTMETABOLITES
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