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Determination of codeine, 6-methoxycodeine and thebaine using capillary electrophoresis with tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection
journal contribution
posted on 1998-01-01, 00:00 authored by Neil BarnettNeil Barnett, B J Hindson, S W Lewis, S D PurcellA simple and robust capillary electrophoresis chemiluminescence detection system for the determination of codeine, 6-methoxycodeine and thebaine is described, based upon the reaction of these analytes with chemically generated tris(2,2′-bipyridyl)ruthenium(III) prepared in sulfuric acid (0.05 M). The reagent solution was contained in a glass detection cell which also held both the capillary and the cathode. The resultant chemiluminescence was monitored directly using a photomultiplier tube mounted flush against the base of the detection cell. The methodology, which incorporated a field amplification sample introduction procedure, realised detection limits (3σ baseline noise) of 5 × 10-8 M for both codeine and 6-methoxycodeine and 1 × 10-7 M for thebaine. The relative standard deviations of the migration times and the peak areas for the three analytes ranged from 2.2 up to 2.5% and 1.9 up to 4.6%, respectively.
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Journal
Analytical communicationsVolume
35Issue
10Pagination
321 - 324Publisher
Royal Society of ChemistryLocation
Cambridge, Eng.Publisher DOI
ISSN
1359-7337Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1998, Royal Society of ChemistryUsage metrics
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