Deakin University
Browse

File(s) under permanent embargo

Determination of codeine, 6-methoxycodeine and thebaine using capillary electrophoresis with tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection

Version 2 2024-06-04, 01:31
Version 1 2019-07-22, 09:12
journal contribution
posted on 2024-06-04, 01:31 authored by Neil BarnettNeil Barnett, BJ Hindson, SW Lewis, SD Purcell
A simple and robust capillary electrophoresis chemiluminescence detection system for the determination of codeine, 6-methoxycodeine and thebaine is described, based upon the reaction of these analytes with chemically generated tris(2,2′-bipyridyl)ruthenium(III) prepared in sulfuric acid (0.05 M). The reagent solution was contained in a glass detection cell which also held both the capillary and the cathode. The resultant chemiluminescence was monitored directly using a photomultiplier tube mounted flush against the base of the detection cell. The methodology, which incorporated a field amplification sample introduction procedure, realised detection limits (3σ baseline noise) of 5 × 10-8 M for both codeine and 6-methoxycodeine and 1 × 10-7 M for thebaine. The relative standard deviations of the migration times and the peak areas for the three analytes ranged from 2.2 up to 2.5% and 1.9 up to 4.6%, respectively.

History

Journal

Analytical communications

Volume

35

Pagination

321-324

Location

Cambridge, Eng.

ISSN

1359-7337

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1998, Royal Society of Chemistry

Issue

10

Publisher

Royal Society of Chemistry

Usage metrics

    Research Publications

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC