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Determination of opiate alkaloids in process liquors using capillary electrophoresis

journal contribution
posted on 2007-02-19, 00:00 authored by B Hindson, Paul FrancisPaul Francis, Stuart Purcell, Neil BarnettNeil Barnett
This paper describes the determination of opiate alkaloids (morphine, codeine, oripavine and thebaine) in industrial process liquors using capillary zone electrophoresis with UV-absorption detection at 214 nm. A study of cyclodextrin type and concentration revealed that the addition of 30 mM hydroxypropyl-β-cyclodextrin to the electrolyte solution (100 mM Tris adjusted to pH 2.8) was suitable to resolve the four analytes of interest. Typical analysis time was 12 min and the limit of detection for each alkaloid was 2.5 × 10−6 M. The results for the proposed methodology were in good agreement with those of a conventional HPLC procedure. Under the same conditions, short-end injection was used to reduce the effective separation length from 41.5 to 8.5 cm, which allowed the determination of morphine and thebaine in process liquors within 2.5 min.


History

Journal

Journal of pharmaceutical and biomedical analysis

Volume

43

Issue

3

Pagination

1164 - 1168

Publisher

Elsevier

Location

New York, NY.

ISSN

0731-7085

eISSN

1873-264X

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2006, Elsevier B.V.