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Determination of sarecycline by UPLC-MS/MS and its application to pharmacokinetic study in rats

journal contribution
posted on 2023-10-10, 05:29 authored by Yonghui Shen, Deru Meng, Feifei ChenFeifei Chen, Hui Jiang, Liming Hu, Yunfang Zhou, Miaomiao Zhang
AbstractSarecycline is a narrow-spectrum antibiotic for the treatment of acne, which is a chronic inflammatory disease of the hair follicle sebaceous glands. In the study, UPLC-MS/MS was used to establish a rapid and accurate analytical method. The sarecycline was determined with poziotinib as internal standard (IS) in rat plasma. An ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm) could performe chromatographic separation with the mobile phase (methanol: water of 0.1% formic acid) with gradient elution. The ions of target fragment were m/z 488.19→410.14 for sarecycline and m/z 492.06→354.55 for poziotinib, which could quantify the electrospray ionization of positive multiple reaction monitoring (MRM) mode. The linear calibration curve of the concentration range was 1–1,000 ng/mL for sarecycline with a lower limit of quantification (LLOQ) of 1 ng/mL. The mean recovery was between 82.46 and 95.85% for sarecycline and poziotinib in rat plasma. RSD for precision of inter-day and intra-day were between 3.24 and 13.36%, and the accuracy ranged from 105.26 to 109.75%. The developed and validated method was perfectly used in the pharmacokinetic study and bioavailability of sarecycline after intravenous and oral administration in rats.

History

Journal

ACTA CHROMATOGRAPHICA

Volume

33

Pagination

228-233

Location

Katowice, Poland

ISSN

1233-2356

eISSN

2083-5736

Language

English

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

3

Publisher

AKADEMIAI KIADO ZRT

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