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Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection

journal contribution
posted on 2005-09-15, 00:00 authored by Jacqui AdcockJacqui Adcock, Neil BarnettNeil Barnett, Jason Wesley Costin, Paul FrancisPaul Francis, S Lewis
The oxidation of selected clinically important neurotransmitter metabolites with acidic potassium permanganate in the presence of polyphosphates evokes chemiluminescence of sufficient intensity to enable the sensitive determination of these species. Limits of detection for 5-hydroxyindole-3-acetic acid (5-HIAA), vanilmandelic acid (VMA; α,4-dihydroxy-3-methoxybenzeneacetic acid), 4-hydroxy-3-methoxyphenylglycol (MHPG), homovanillic acid (HVA, 4-hydroxy-3-methoxyphenylacetic acid) and 3,4-dihydroxyphenylacetic acid (DOPAC) were between 5 × 10−9 and 4 × 10−8 M, using flow-injection analysis methodology. In addition, we demonstrate the rapid determination of homovanillic acid and 5-hydroxyindole-3-acetic acid in human urine – without the need for extraction procedures – using monolithic column chromatography with chemiluminescence detection.

History

Journal

Talanta

Volume

67

Issue

3

Pagination

585 - 589

Publisher

Elsevier BV

Location

Amsterdam, Netherlands

ISSN

0039-9140

eISSN

1873-3573

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2005, Elsevier BV