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Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection
journal contribution
posted on 2005-09-15, 00:00 authored by Jacqui AdcockJacqui Adcock, Neil BarnettNeil Barnett, Jason Wesley Costin, Paul FrancisPaul Francis, S LewisThe oxidation of selected clinically important neurotransmitter metabolites with acidic potassium permanganate in the presence of polyphosphates evokes chemiluminescence of sufficient intensity to enable the sensitive determination of these species. Limits of detection for 5-hydroxyindole-3-acetic acid (5-HIAA), vanilmandelic acid (VMA; α,4-dihydroxy-3-methoxybenzeneacetic acid), 4-hydroxy-3-methoxyphenylglycol (MHPG), homovanillic acid (HVA, 4-hydroxy-3-methoxyphenylacetic acid) and 3,4-dihydroxyphenylacetic acid (DOPAC) were between 5 × 10−9 and 4 × 10−8 M, using flow-injection analysis methodology. In addition, we demonstrate the rapid determination of homovanillic acid and 5-hydroxyindole-3-acetic acid in human urine – without the need for extraction procedures – using monolithic column chromatography with chemiluminescence detection.
History
Journal
TalantaVolume
67Issue
3Pagination
585 - 589Publisher
Elsevier BVLocation
Amsterdam, NetherlandsPublisher DOI
ISSN
0039-9140eISSN
1873-3573Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2005, Elsevier BVUsage metrics
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No categories selectedKeywords
neurotransmitter metabolitesmonolithic column chromatographychemiluminescence detectionScience & TechnologyPhysical SciencesChemistry, AnalyticalChemistryPERFORMANCE LIQUID-CHROMATOGRAPHYACIDIC POTASSIUM-PERMANGANATEFLOW-INJECTION ANALYSISDERIVATIZATION REAGENTPRECOLUMN DERIVATIZATIONCATECHOLAMINES6-AMINOMETHYLPHTHALHYDRAZIDE5-HYDROXYINDOLESSEROTONINPLASMA
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