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Determination of urea using high-performance liquid chromatography with fluorescence detection after automated derivatisation with Xanthydrol
journal contribution
posted on 2007-08-17, 00:00 authored by S Clark, Paul FrancisPaul Francis, Xavier ConlanXavier Conlan, Neil BarnettNeil BarnettA high-performance liquid chromatography (HPLC) method for the determination of urea that incorporates automated derivatisation with xanthydrol (9H-xanthen-9-ol) is described. Unlike the classic xanthydrol approach for the determination of urea, which involves the precipitation of dixanthylurea (N,N′-di-9H-xanthen-9-ylurea), the derivatisation procedure employed in this method produces N-9H-xanthen-9-ylurea, which remains in solution and can be quantified using fluorescence detection (λex = 213 nm; λem = 308 nm) after chromatographic separation from interferences. The limit of detection for urea was 5 × 10−8 M (0.003 mg L−1). This method was applied to the determination of urea in human and animal urine and in wine.
History
Journal
Journal of chromatography AVolume
1161Issue
1-2Pagination
207 - 213Publisher
Elsevier BVLocation
Amsterdam, NetherlandsPublisher DOI
ISSN
0021-9673eISSN
1873-3778Language
engPublication classification
C1 Refereed article in a scholarly journal; C Journal articleCopyright notice
2007, Elsevier B.V.Usage metrics
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HPLCureaxanthydrolfluorescence derivatisationmass spectrometryScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryANALYTICAL METHODOLOGYETHYL CARBAMATESOIL EXTRACTSBEVERAGESALLANTOINNITROGENREAGENTSAMPLESPLASMABLOOD
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