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Development of Cotton leaf curl virus resistant transgenic cotton using antisense beta C1 gene
journal contribution
posted on 2023-05-21, 23:34 authored by Sayed Sartaj Sohrab, Mohammad A Kamal, Abdul Ilah, Azamal Husen, Prasad BhattacharyaPrasad Bhattacharya, D RanaCotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization. The developed transgenic and inoculated plants remained symptomless till their growth period. In conclusion, the plants were observed as resistant to CLCuV.
History
Journal
SAUDI JOURNAL OF BIOLOGICAL SCIENCESVolume
23Pagination
358-362Location
Saudi ArabiaPublisher DOI
ISSN
1319-562XeISSN
2213-7106Language
EnglishPublication classification
C1.1 Refereed article in a scholarly journalIssue
3Publisher
ELSEVIER SCIENCE BVUsage metrics
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No categories selectedKeywords
Science & TechnologyLife Sciences & BiomedicineBiologyLife Sciences & Biomedicine - Other TopicsTransgenic cotton plantsCLCuV resistanceGene transferMolecular verificationVirus resistanceRNA INTERFERENCEDISEASESATELLITETOMATOTHREATA. tumefaciens, Agrobacterium tumefaciensCLCuV, Cotton leaf curl virusCP, coat proteinMS, Murashige and Skoog’sPCR, polymerase chain reactionPDR, pathogen derived resistanceBiotechnology
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