Deakin University
Browse

File(s) under permanent embargo

Development of a microsatellite-based method for the differentiation of European wild boar (Sus scrofa scrofa) from domestic pig breeds (Sus scrofa domestica) in food

journal contribution
posted on 2012-04-01, 00:00 authored by C M Conyers, Theodore Allnutt, H J Hird, J Kaye, J Chisholm
Twenty microsatellites (simple sequence repeats, SSR) were used to discriminate wild boar from domestic pig and to identify mixtures of the two. Reference groups of wild boar and pig samples were collected from the UK and Europe for genetic assignment tests. Bayesian Analysis of Populations software (BAPs) gave 100% correct assignment for blind wild boar and pig samples and correctly identified mixed samples. DNA was extracted from 12 commercial food samples (11 labeled as containing wild boar) including patés, salamis, and sausage, and good SSR profiles were obtained. Eleven samples were correctly assigned as pig, and two as mixed meats. One sample sold as wild boar meat was clearly assigned as pig. A further 10 blind samples of meat cuts were analyzed, eight wild boar and two pig, and all were correctly assigned.

History

Journal

Journal of agricultural and food chemistry

Volume

60

Issue

13

Pagination

3341 - 3347

Publisher

American Chemical Society

Location

Washington, D.C.

ISSN

0021-8561

eISSN

1520-5118

Language

eng

Publication classification

C Journal article; C1.1 Refereed article in a scholarly journal

Copyright notice

2012, American Chemical Society