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Development of a quantitative immunodipstick assay for Phytophthora nicotianae

Version 2 2024-06-03, 10:17
Version 1 2015-07-23, 15:54
journal contribution
posted on 1999-09-01, 00:00 authored by Y Gautam, David CahillDavid Cahill, A R Hardham
Monoclonal antibodies have been produced that are specific for the species, Phytophthora nicotianae, a plant pathogen which causes blackshank on tobacco and root rots in citrus. The antibodies react with the surface of P. nicotianae cysts but not with cysts of any other species of Phytophthora or Pythium tested. The antibodies have been used in a dipstick assay in which a colorimetric detection system gives a quantitative indication of the level of inoculum present. In this assay, one of the species-specific antibodies, Pn8E6, detected P. nicotianae spores at densities as low as 10 spores/ml. In preliminary tests, the assay was able to detect P. nicotianae in contaminated soil samples and the intensity of the reaction showed a correlation with results obtained from plating assays on selective media.

History

Journal

Food and Agricultural Immunology

Volume

11

Issue

3

Pagination

229 - 242

Publisher

Taylor & Francis

Location

Abingdon, Eng.

ISSN

0954-0105

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1999, Taylor & Francis