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Development of a stable continuous flow immobilized enzyme reactor for the hydrolysis of inulin

journal contribution
posted on 2008-07-01, 00:00 authored by Munish PuriMunish Puri, R Singh, R Dhaliwal
A 23.5-fold purified exoinulinase with a specific activity of 413 IU/mg and covalently immobilized on Duolite A568 has been used for the development of a continuous flow immobilized enzyme reactor for the hydrolysis of inulin. In a packed bed reactor containing 72 IU of exoinulinase from Kluyveromyces marxianus YS-1, inulin solution (5%, pH 5.5) with a flow rate of 4 mL/h was completely hydrolyzed at 55 °C. The reactor was run continuously for 75 days and its experimental half-life was 72 days under the optimized operational conditions. The volumetric productivity and fructose yield of the reactor were 44.5 g reducing sugars/L/h and 53.3 g/L, respectively. The hydrolyzed product was a mixture of fructose (95.8%) and glucose (4.2%) having an average fructose/glucose ratio of 24. An attempt has also been made to substitute pure inulin with raw Asparagus racemosus inulin to determine the operational stability of the developed reactor. The system remained operational only for 11 days, where 85.9% hydrolysis of raw inulin was achieved.

History

Journal

Journal of industrial microbiology & biotechnology

Volume

35

Issue

7

Pagination

777 - 782

Publisher

Springer

Location

Berlin, Germany

ISSN

1367-5435

eISSN

1476-5535

Language

eng

Notes

Published online: 4 April 2008

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2008, Society for Industrial Microbiology