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Development of drug-loaded chitosan-vanillin nanoparticles and its cytotoxicity against HT-29 cells

Version 2 2024-06-06, 02:59
Version 1 2016-05-13, 09:39
journal contribution
posted on 2024-06-06, 02:59 authored by P-W Li, G Wang, Z-M Yang, Wei DuanWei Duan, Z Peng, Lingxue KongLingxue Kong, Q-H Wang
Chitosan as a natural polysaccharide derived from chitin of arthropods like shrimp and crab, attracts much interest due to its inherent properties, especially for application in biomedical materials. Presently, biodegradable and biocompatible chitosan nanoparticles are attractive for drug delivery. However, some physicochemical characteristics of chitosan nanoparticles still need to be further improved in practice. In this work, chitosan nanoparticles were produced by crosslinking chitosan with 3-methoxy-4-hydroxybenzaldehyde (vanillin) through a Schiff reaction. Chitosan nanoparticles were 200-250 nm in diameter with smooth surface and were negatively charged with a zeta potential of - 17.4 mV in neutral solution. Efficient drug loading and drug encapsulation were achieved using 5-fluorouracil as a model of hydrophilic drug. Drug release from the nanoparticles was constant and controllable. The in vitro cytotoxicity against HT-29 cells and cellular uptake of the chitosan nanoparticles were evaluated by methyl thiazolyl tetrazolium method, confocal laser scanning microscope and flow cytometer, respectively. The results indicate that the chitosan nanoparticles crosslinked with vanillin are a promising vehicle for the delivery of anticancer drugs.

History

Journal

Drug delivery

Volume

23

Pagination

30-35

Location

Abingdon, Eng.

ISSN

1071-7544

eISSN

1521-0464

Language

eng

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2014, Informa UK

Issue

1

Publisher

Taylor & Francis