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Differential effects of exercise on insulin-signaling gene expression in human skeletal muscle.
journal contribution
posted on 2001-02-01, 00:00 authored by Glenn WadleyGlenn Wadley, R Tunstall, A Sanigorski, Gregory Collier, Mark Hargreaves, David Cameron-SmithSkeletal muscle insulin sensitivity is enhanced after acute exercise and short-term endurance training. We investigated the impact of exercise on the gene expression of key insulin-signaling proteins in humans. Seven untrained subjects (4 women and 3 men) completed 9 days of cycling at 63 ± 2% of peak O2 uptake for 60 min/day. Muscle biopsies were taken before, immediately after, and 3 h after the exercise bouts (on days 1 and 9). The gene expression of insulin receptor substrate-2 and the p85α subunit of phosphatidylinositol 3-kinase was significantly higher 3 h after a single exercise bout, although short-term training ameliorated this effect. Gene expression of insulin receptor and insulin receptor substrate-1 was not significantly altered at any time point. These results suggest that exercise may have a transitory impact on the expression of insulin receptor substrate-2 and phosphatidylinositol 3-kinase; however, the predominant actions of exercise on insulin sensitivity appear not to reside in the transcriptional activation of the genes encoding major insulin-signaling proteins.
History
Journal
Journal of applied physiologyVolume
90Issue
2Pagination
436 - 440Publisher
American Physiological SocietyLocation
Bethesda, Md.ISSN
8750-7587eISSN
1522-1601Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2001, American Physiological SocietyUsage metrics
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messenger ribonucleic acidreal-time polymerase chain reactiontranscriptioninsulin-receptor substratesphosphatidylinositol 3-kinaseScience & TechnologyLife Sciences & BiomedicinePhysiologySport SciencesDEPENDENT DIABETES-MELLITUSRECEPTOR SUBSTRATE-2GLUCOSE-TRANSPORTPHYSICAL-ACTIVITYTERM EXERCISEIRS-2GLUT-4TRANSDUCTIONADAPTATION
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