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Distinct gut virome profile of pregnant women with type 1 diabetes in the ENDIA study

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journal contribution
posted on 2019-02-01, 00:00 authored by Ki Wook Kim, Digby W Allen, Thomas Briese, Jennifer J Couper, Simon C Barry, Peter G Colman, Andrew M Cotterill, Elizabeth A Davis, Lynne C Giles, Leonard C Harrison, Mark Harris, Aveni Haynes, Jessica L Horton, Sonia R Isaacs, Komal Jain, Walter Ian Lipkin, Grant Morahan, Claire Morbey, Ignatius C N Pang, Anthony T Papenfuss, Megan A S Penno, Richard O Sinnott, Georgia Soldatos, Rebecca L Thomson, Peter VuillerminPeter Vuillermin, John M Wentworth, Marc R Wilkins, William D Rawlinson, Maria E Craig, ENDIA Study Group
Background: The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture sequencing to characterize the gut virome of pregnant women with and without type 1 diabetes (T1D), longitudinally followed in the Environmental Determinants of Islet Autoimmunity study. Methods: In total, 61 pregnant women (35 with T1D and 26 without) from Australia were examined. Nucleic acid was extracted from serial fecal specimens obtained at prenatal visits, and viral genomes were sequenced by virome capture enrichment. The frequency, richness, and abundance of viruses were compared between women with and without T1D. Results: Two viruses were more prevalent in pregnant women with T1D: picobirnaviruses (odds ratio [OR], 4.2; 95% confidence interval [CI], 1.0-17.1; P = .046) and tobamoviruses (OR, 3.2; 95% CI, 1.1-9.3; P = .037). The abundance of 77 viruses significantly differed between the 2 maternal groups (≥2-fold difference; P < .02), including 8 Enterovirus B types present at a higher abundance in women with T1D. Conclusions: These findings provide novel insight into the composition of the gut virome during pregnancy and demonstrate a distinct profile of viruses in women with T1D.

History

Journal

Open forum infectious diseases

Volume

6

Issue

2

Article number

ofz025

Pagination

1 - 8

Publisher

Oxford University Press

Location

Oxford, Eng.

ISSN

2328-8957

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2019, The Author(s)