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Disturbed mitochondrial function restricts glutamate uptake in the human Müller glia cell line, MIO-M1

journal contribution
posted on 2017-09-01, 00:00 authored by R Vohra, I S Gurubaran, U Henriksen, L H Bergersen, Lene Rasmussen, C Desler, D M Skytt, M Kolko
Using the human Müller cell line, MIO-M1, the aim was to study the impact of mitochondrial inhibition in Müller glia through antimycin A treatment. MIO-M1 cell survival, levels of released lactate, mitochondrial function, and glutamate uptake were studied in response to mitochondrial inhibition and glucose restriction. Lactate release decreased in response to glucose restriction. Combined glucose restriction and blocked mitochondrial activity decreased survival and caused collapse of the respiratory chain measured by oxygen consumption rate and extracellular acidification rate. Mitochondrial inhibition caused impaired glutamate uptake and decreased mRNA expression of the glutamate transporter, EAAT1. Over all, we show important roles of mitochondrial activity in MIO-M1 cell function and survival.

History

Journal

Mitochondrion

Volume

36

Pagination

52 - 59

ISSN

1567-7249

eISSN

1872-8278