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DryLab® optimised two-dimensional high performance liquid chromatography for differentiation of ephedrine and pseudoephedrine based methamphetamine samples

Version 2 2024-06-06, 09:26
Version 1 2015-03-10, 15:12
journal contribution
posted on 2024-06-06, 09:26 authored by LM Andrighetto, PG Stevenson, JR Pearson, Luke HendersonLuke Henderson, Xavier ConlanXavier Conlan
In-silico optimised two-dimensional high performance liquid chromatographic (2D-HPLC) separations of a model methamphetamine seizure sample are described, where an excellent match between simulated and real separations was observed. Targeted separation of model compounds was completed with significantly reduced method development time. This separation was completed in the heart-cutting mode of 2D-HPLC where C18 columns were used in both dimensions taking advantage of the selectivity difference of methanol and acetonitrile as the mobile phases. This method development protocol is most significant when optimising the separation of chemically similar chemical compounds as it eliminates potentially hours of trial and error injections to identify the optimised experimental conditions. After only four screening injections the gradient profile for both 2D-HPLC dimensions could be optimised via simulations, ensuring the baseline resolution of diastereomers (ephedrine and pseudoephedrine) in 9.7 min. Depending on which diastereomer is present the potential synthetic pathway can be categorized.

History

Journal

Forensic science international

Volume

244

Pagination

302-305

Location

Shannon, Ireland

ISSN

0379-0738

eISSN

1872-6283

Language

eng

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2014, Elsevier Ireland

Publisher

Elsevier Ireland

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