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Dual-biomarker-based logic-controlled electrochemical diagnosis for prostate cancers

Version 2 2024-06-13, 12:53
Version 1 2019-03-14, 12:17
journal contribution
posted on 2024-06-13, 12:53 authored by J Liu, H Zhou, JJ Xu, HY Chen
As single biomarker often has inadequate predictive value in clinical examination, we designed a dual-biomarker "AND" logic device that defines the concentration of prostate-specific antigen (PSA) and survivin mRNA as inputs and the increase in faradaic current from intercalation agent Co(phen) 33+ as output for prostate cancer (CaP) diagnosis on Au nano-film covered gold electrode surfaces. Primary capture antibody (Ab 1 ) was first immobilized on AuNP electrode, then while both PSA and survivin mRNA exist, current of Co(phen) 33+ observably increased due to the formation of dsDNA structure between survivin antisensoligonucleotide connected secondary detection antibody (Ab 2 ) and survivin mRNA. And when one of the two biomarkers was absent, no obvious current increment was found. Thus the designed "AND" logic gate greatly reduced the possibility of false-positive assay from non-cancerous prostate diseases such as prostatic hyperplasia and prostatitis in diagnosis of CaPs. © 2013 2013 Elsevier B.V. All rights reserved.

History

Journal

Electrochemistry communications

Volume

32

Pagination

27-30

Location

Amsterdam, The Netherlands

ISSN

1388-2481

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2013, Elsevier B.V.

Publisher

Elsevier

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