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Dual-biomarker-based logic-controlled electrochemical diagnosis for prostate cancers

journal contribution
posted on 2013-07-01, 00:00 authored by Jing Liu, H Zhou, J J Xu, H Y Chen
As single biomarker often has inadequate predictive value in clinical examination, we designed a dual-biomarker "AND" logic device that defines the concentration of prostate-specific antigen (PSA) and survivin mRNA as inputs and the increase in faradaic current from intercalation agent Co(phen) 33+ as output for prostate cancer (CaP) diagnosis on Au nano-film covered gold electrode surfaces. Primary capture antibody (Ab 1 ) was first immobilized on AuNP electrode, then while both PSA and survivin mRNA exist, current of Co(phen) 33+ observably increased due to the formation of dsDNA structure between survivin antisensoligonucleotide connected secondary detection antibody (Ab 2 ) and survivin mRNA. And when one of the two biomarkers was absent, no obvious current increment was found. Thus the designed "AND" logic gate greatly reduced the possibility of false-positive assay from non-cancerous prostate diseases such as prostatic hyperplasia and prostatitis in diagnosis of CaPs. © 2013 2013 Elsevier B.V. All rights reserved.

History

Journal

Electrochemistry communications

Volume

32

Pagination

27 - 30

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

1388-2481

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2013, Elsevier B.V.

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