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Effect of 2 weeks of endurance training on uncoupling protein3 content in untrained human subjects
journal contribution
posted on 2005-03-01, 00:00 authored by P Schrauwen, Aaron RussellAaron Russell, E Moonen-Kornips, N Boon, M HesselinkAim: The mitochondrial uncoupling protein-3 (UCP3) is able to lower the proton gradient across the inner mitochondrial membrane, thereby uncoupling substrate oxidation from ATP production and dissipating energy as heat. What the effect of endurance training on UCP3 is, is still controversial. Endurance-trained athletes are characterized by lower levels of UCP3, but longitudinal studies in rodents reported no effect of endurance training on muscular UCP3 levels. Here, we examined the effect of a 2-week training programme on skeletal muscle UCP3 protein content in untrained human subjects, and hypothesized that UCP3 will be reduced after the training programme. Methods: Nine untrained men [age: 23.3±3.2 years; BMI: 22.6±2.6 kg m-2; maximal power output (Wmax): 3.8±0.6 W kg-1 body weight] trained for 2 weeks. Before and at least 72 h after the training period, muscle biopsies were taken for determination of UCP3 protein content. Results: UCP3 protein content tended to be lower after the training programme [95±10 vs. 109±12 arbitrary units (AU), P= 0.08]. Cytochrome c content tended to increase with 33% in response to endurance training (52± 6 vs. 39± 6 AU, P = 0.08). The ratio UCP3 relative to cytochrome c tended to decrease significantly upon endurance training (2.0±0.4 vs. 3.2±0.6 AU, P = 0.01). Conclusion: A short-term (2-week) endurance training programme decreased UCP3 protein levels and significantly reduced the ratio of UCP3 to cytochrome c.
History
Journal
Acta physiologicaVolume
183Issue
3Pagination
273 - 280Publisher
Blackwell Publishing LtdLocation
Oxford, EnglandISSN
1748-1708eISSN
1748-1716Language
engPublication classification
C1.1 Refereed article in a scholarly journal; C Journal articleCopyright notice
2005, Scandinavian Physiological SocietyUsage metrics
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