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Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle

journal contribution
posted on 2004-09-01, 00:00 authored by G Kraniou, David Cameron-Smith, Mark Hargreaves
Six untrained, male subjects (23 ± 1 years old, 84 ± 5 kg, VO2peak= 3.7 ± 0.8 l min–1) exercised for 60 min at 75 ± 1%VO2peak on 7 consecutive days.  Muscle samples were obtained before the start of cycle exercise training and 24 h after the first and seventh exercise sessions and analysed for citrate synthase activity, glycogen and glucose transporter 4 (GLUT-4) mRNA and protein expression. Exercise training increased (P < 0.05) citrate synthase by ~20% and muscle glycogen concentration by ~40%. GLUT-4 mRNA levels 24 h after the first and seventh exercise sessions were similar to those  measured before the start of exercise training. In contrast, GLUT-4 protein expression was increased after 7 days of exercise training (12.4 ± 1.5 versus 3.4 ± 1.0 arbitray units (a.u.), P < 0.05) and although it tended to be higher 24 h after the first exercise session (6.0 ± 3.0 versus 3.4 ± 1.0 a.u.), this was not significantly different (P= 0.09). These results support the suggestion that the adaptive increase in skeletal muscle GLUT-4 protein expression with short-term exercise training arises from the repeated, transient increases in GLUT-gene transcription following each exercise bout leading to a gradual accumulation of GLUT-4 protein, despite GLUT-4 mRNA returning to basal levels between exercise stimuli.

History

Journal

Experimental physiology

Volume

89

Issue

5

Pagination

559 - 563

Publisher

Cambridge University Press for the Physiological Society

Location

Cambridge, England

ISSN

0958-0670

eISSN

1469-445X

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2004, The Physiological Society