Deakin University
Browse
rivera-effectsoffood-2016.pdf (1.73 MB)

Effects of food components that activate TRPA1 receptors on mucosal ion transport in the Mouse intestine

Download (1.73 MB)
Version 2 2024-05-31, 05:08
Version 1 2017-03-03, 14:45
journal contribution
posted on 2024-05-31, 05:08 authored by LJ Fothergill, B Callaghan, Leni RiveraLeni Rivera, TM Lieu, DP Poole, HJ Cho, DM Bravo, John FurnessJohn Furness
TRPA1 is a ligand-activated cation channel found in the intestine and other tissues. Components of food that stimulate TRPA1 receptors (phytonutrients) include allyl isothiocyanate, cinnamaldehyde and linalool, but these may also act at other receptors. Cells lining the intestinal mucosa are immunoreactive for TRPA1 and Trpa1 mRNA occurs in mucosal extracts, suggesting that the TRPA1 receptor is the target for these agonists. However, in situ hybridisation reveals Trpa1 expression in 5-HT containing enteroendocrine cells, not enterocytes. TRPA1 agonists evoke mucosal secretion, which may be indirect (through release of 5-HT) or direct by activation of enterocytes. We investigated effects of the phytonutrients on transmucosal ion currents in mouse duodenum and colon, and the specificity of the phytonutrients in cells transfected with Trpa1, and in Trpa1-deficient mice. The phytonutrients increased currents in the duodenum with the relativepotencies: allyl isothiocyanate (AITC) > cinnamaldehyde > linalool (0.1 to 300 µM). The rank order was similar in the colon, but linalool was ineffective. Responses to AITC were reduced by the TRPA1 antagonist HC-030031 (100 µM), and were greatly diminished in Trpa1-/- duodenum and colon. Responses were not reduced by tetrodotoxin, 5-HT receptor antagonists, or atropine, but inhibition of prostaglandin synthesis reduced responses. Thus, functional TRPA1 channels are expressed by enterocytes of the duodenum and colon. Activation of enterocyte TRPA1 by food components has the potential to facilitate nutrient absorption.

History

Journal

Nutrients

Volume

8

Article number

ARTN 623

Location

Switzerland

ISSN

2072-6643

eISSN

2072-6643

Language

English

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2016, by the authors

Issue

10

Publisher

MDPI AG