owens-effectsofplacental-2000.pdf (488.84 kB)
Effects of placental insufficiency on the ovine fetal renin-angiotensin system
journal contribution
posted on 2000-01-01, 00:00 authored by D Y Zhang, E R Lumbers, G Simonetta, J J Wu, Julie Owens, J S Robinson, I C McMillenWe postulated that chronic placental insufficiency would be associated with reduced expression of renal renin and angiotensinogen genes in the fetal sheep. Placental development was restricted in ewes by removing the majority of caruncles prior to mating (placentally restricted (PR) group). The weights of PR fetuses were significantly reduced (P < 0.05, 2.98 +/- 0.33 kg) compared to control fetuses (4.20 +/- 0.30 kg). Kidney weights were also significantly reduced in the PR fetuses (P < 0.05, 8.4 +/- 0.9 g) compared with control fetuses (12.2 +/- 1.3 g). The ratios of renal renin/-actin mRNA levels were significantly reduced in PR fetuses (P < 0.001, 0.35 +/- 0.02) when compared to control animals (0.98 +/- 0.13). The renal angiotensinogen mRNA/18S rRNA ratio was significantly lower (P < 0.05, 0.28 +/- 0.13) in PR fetuses compared with control fetuses (0.72 +/- 0.10), while hepatic angiotensinogen was unaffected. There was a positive correlation between renal renin mRNA and renal angiotensinogen mRNA levels (r = 0.65, P < 0.05, n = 12). It is unlikely that these changes in renal angiotensinogen and renin mRNA were due to the small increment in plasma cortisol levels (< 5 nmol l-1). There was, however, a positive correlation between arterial PO2 and renal renin mRNA (r2 = 0.77, P < 0.01). Plasma renin levels were not different between the two groups. Thus, restriction of nutrient and oxygen supply throughout fetal life was associated with suppression of renal renin and renal angiotensinogen gene expression, with no effect on hepatic angiotensinogen mRNA levels. This specific suppression of fetal renal renin and angiotensinogen expression could alter the activity of the intrarenal RAS and so affect growth and development of the kidney.
History
Journal
Experimental physiologyVolume
85Issue
1Pagination
79 - 84Publisher
The Physiological SocietyLocation
London, Eng.Publisher DOI
Link to full text
ISSN
0958-0670Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2000, The Physiological SocietyUsage metrics
Keywords
AngiotensinogenAnimalsAutoradiographyBody WeightFemaleFetusHydrocortisoneLiverOrgan SizeOxygen ConsumptionPlacental InsufficiencyPregnancyRNA, MessengerReninRenin-Angiotensin SystemRibonucleasesSheepScience & TechnologyLife Sciences & BiomedicinePhysiologyINTRAUTERINE GROWTH-RETARDATIONCONVERTING ENZYMECARDIOVASCULAR-DISEASEGENE-EXPRESSIONRENAL-FUNCTIONFACTOR-IRESTRICTIONKIDNEYMICEPhysiology
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