Deakin University
Browse

File(s) under permanent embargo

Electrochemical detection of mismatched DNA using a MutS probe

journal contribution
posted on 2023-02-06, 01:44 authored by M Cho, S Lee, S Y Han, J Y Park, Md Aminur Rahman, Y B Shim, C Ban
A direct and label-free electrochemical biosensor for the detection of the protein-mismatched DNA interaction was designed using immobilized N-terminal histidine tagged Escherichia coli. MutS on a Ni-NTA coated Au electrode. General electrochemical methods, cyclic voltammetry (CV), electrochemical quartz crystal microbalance (EQCM) and impedance spectroscopy, were used to ascertain the binding affinity of mismatched DNAs to the MutS probe. The direct results of CV and impedance clearly reveal that the interaction of MutS with the CC heteroduplex was much stronger than that with AT homoduplex, which was not differentiated in previous results (GT > CT > CC ≈ AT) of a gel mobility shift assay. The EQCM technique was also able to quantitatively analyze MutS affinity to heteroduplexes. © 2006 Oxford University Press.

History

Journal

Nucleic Acids Research

Volume

34

ISSN

0305-1048

eISSN

1362-4962

Usage metrics

    Research Publications

    Categories

    No categories selected

    Keywords

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC