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Elevated serum phosphatidylcholine (16:1/22:6) levels promoted by fish oil and vitamin D3 are highly correlated with biomarkers of non-alcoholic fatty liver disease in Chinese subjects
journal contributionposted on 2023-11-21, 04:45 authored by ZK Fan, WJ Ma, W Zhang, H Li, J Zhai, T Zhao, XF Guo, Andrew SinclairAndrew Sinclair, D Li
The aim of the present study was to investigate the relationship between the changes of serum lipid metabolites and the risk factors of non-alcoholic fatty liver disease (NAFLD) after fish oil (FO) or fish oil plus vitamin D (FO + D) intervention in Chinese NAFLD subjects. Seventy-four NAFLD subjects, aged 55.2 ± 15.9 years, were randomized to consume FO + D (n = 23), FO (n = 27) or corn oil (CO, n = 24) capsules for a 3-month intervention. Serum lipid-related metabolites were measured with ultraperformance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomics approach together with multivariate data analysis. The differential metabolites were screened and identified with variable importance in projection (VIP) scores based on orthogonal partial least squares discriminant analysis models. Serum phosphatidylcholine (PC) (16:1/22:6) levels had the highest and second highest VIP scores following FO + D and FO interventions, respectively. Serum PC (16:1/22:6) levels were negatively correlated with circulating alanine transaminase (ALT) (r = −0.268, p = 0.021), triacylglycerol (TAG) (r = −0.236, p = 0.042), interleukin (IL)-1β (r = −0.401, p < 0.001) and tumor necrosis factor (TNF)-α (r = −0.322, p = 0.005) concentrations, and were positively correlated with high-density lipoprotein cholesterol (HDL-C) (r = 0.272, p = 0.019) concentrations. The present study was the first to report that serum PC (16:1/22:6) levels were highly correlated with ALT, TAG, HDL-C, IL-1β and TNF-α concentrations, indicating that PC (16:1/22:6) might ameliorate lipid metabolism and inflammation in NAFLD subjects.
JournalFood and Function
PublisherROYAL SOC CHEMISTRY
Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyFood Science & TechnologyENRICHED PHOSPHATIDYLCHOLINEINSULIN-RESISTANCEACIDSEPAMECHANISMSPLASMACELLSRICHHumansFish OilsNon-alcoholic Fatty Liver DiseaseCholecalciferolPhosphatidylcholinesBiomarkersTriglyceridesCholesterol, HDLTumor Necrosis Factor-alphaAlanine TransaminaseChinaPreventionLiver DiseaseNutritionDigestive DiseasesComplementary and Integrative HealthChronic Liver Disease and Cirrhosis2.1 Biological and endogenous factors2 AetiologyInflammatory and immune system