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Enhanced polycyclic aromatic hydrocarbons degradation in rhizosphere soil planted with tall fescue: bacterial community and functional gene expression mechanisms
journal contributionposted on 2018-12-01, 00:00 authored by Meixia Guo, Zongqiang Gong, Renhui Miao, Chunyun Jia, Jim RookesJim Rookes, David CahillDavid Cahill, Jie Zhuang
To investigate the bacterial mechanisms of polycyclic aromatic hydrocarbons (PAHs) degradation in an aged-contaminated agricultural soil planted with tall fescue (Festuca arundinacea), a rhizo-box experiment was carried out for 60 d. Shifts in bacterial community structure in the soils during the experiment were performed using denaturing gradient gel electrophoresis. The abundance and activity of total bacteria and PAH-degraders were measured by quantification of 16S rDNA, PAH-ring hydroxylating dioxygenase (PAH-RHDα) genes and their transcripts, respectively. The residual PAH concentrations were monitored using high-performance liquid chromatography analysis. Results showed that the removal percentage of total PAHs in rhizosphere soil was 11% higher than that in unplanted soil. Soil bacteria were dominated by Alphaproteobacteria (48.4%) and Gammaproteobacteria (25.8%). Tall fescue positively affected the abundance and activity of total bacteria in the soil, and stimulated RHDα gram-negative (GN) gene expression while inhibiting RHDα gram-positive gene expression. PAH dissipation in rhizosphere soil could be ascribed to modifications in the bacterial community structure, increase in the abundance of PAH-degraders, and enhancement of the RHDα GN gene expression during the incubation.
Pagination15 - 23
LocationAmsterdam, The Netherlands
Publication classificationC Journal article; C1 Refereed article in a scholarly journal
Copyright notice2018, Elsevier Ltd.
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Polycyclic aromatic hydrocarbonsRhizosphere effectRHDα gene expressionBacterial communityPhytoremediationScience & TechnologyLife Sciences & BiomedicineEnvironmental SciencesEnvironmental Sciences & EcologyRHD alpha gene expressionRING-HYDROXYLATING DIOXYGENASESPAH-CONTAMINATED SOILBIOREMEDIATIONDIVERSITYPCRBIOAVAILABILITYBIODEGRADATIONQUANTIFICATIONPHENANTHRENE