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Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices

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Version 1 2015-02-05, 12:37
journal contribution
posted on 2024-06-04, 04:57 authored by ZM Smith, JM Terry, Neil BarnettNeil Barnett, Laura GrayLaura Gray, DJ Wright, Paul FrancisPaul Francis
Acidic potassium permanganate chemiluminescence enables direct post-column detection of glutathione, but its application to assess the redox state of a wider range of biological fluids and tissues is limited by its sensitivity. Herein we show that the simple on-line addition of an aqueous formaldehyde solution not only enhances the sensitivity of the procedure by two orders of magnitude, but also provides a remarkable improvement in the selectivity of the reagent towards thiols such as glutathione (compared to phenols and amino acids that do not possess a thiol group). This enhanced mode of detection was applied to the determination of glutathione and its corresponding disulfide species in homogenised striatum samples taken from both wild type mice and the R6/1 transgenic mouse model of Huntington's disease, at both 8 and 12 weeks of age. No significant difference was observed between the GSH/GSSG ratios of wild type mice and R6/1 mice at either age group, suggesting that the early disease progression had not significantly altered the intracellular redox environment.

History

Journal

Analyst

Volume

139

Pagination

2416-2422

Location

London, Eng.

Open access

  • Yes

eISSN

1364-5528

Language

eng

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2014, Royal Society of Chemistry

Issue

10

Publisher

Royal Society of Chemistry