Deakin University
Browse
verma-enzymaticsynthesis-2008.pdf (93.03 kB)

Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372

Download (93.03 kB)
journal contribution
posted on 2008-09-01, 00:00 authored by Madan Verma, G Chauhan, S Kanwar
A purified alkaline thermo-tolerant bacterial lipase from Bacillus cereus MTCC 8372 was immobilized on a Poly (MAc- co -DMA- cl -MBAm) hydrogel. The hydrogel showed approximately 94% binding capacity for lipase. The immobilized lipase (2.36 IU) was used to achieve esterification of myristic acid and isopropanol in n -heptane at 65 °C under continuous shaking. The myristic acid and isopropanol when used at a concentration of 100 mM each in n -heptane resulted in formation of isopropyl myristate (66.0 ± 0.3 mM) in 15 h. The reaction temperature below or higher than 65°C markedly reduced the formation of isopropyl myristate. Addition of a molecular sieve (3 Å × 1.5 mm) to the reaction mixture drastically reduced the ester formation. The hydrogel bound lipase when repetitively used to perform esterification under optimized conditions resulted in 38.0 ± 0.2 mM isopropyl myristate after the 3 rd cycle of esterification.

History

Journal

Acta microbiologica et immunologica hungarica

Volume

55

Issue

3

Pagination

327 - 342

Publisher

Akadémiai Kiadó

Location

Budapest, Hungary

ISSN

1217-8950

eISSN

1588-2640

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2008, Akadémiai Kiadó

Usage metrics

    Research Publications

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC