Deakin University
Browse

File(s) under permanent embargo

Estimating the in-vivo HIV template switching and recombination rate

Version 2 2024-06-13, 09:36
Version 1 2016-01-11, 10:45
journal contribution
posted on 2024-06-13, 09:36 authored by D Cromer, AJ Grimm, TE Schlub, J Mak, MP Davenport
BACKGROUND: HIV recombination has been estimated in vitro using a variety of approaches, and shows a high rate of template switching per reverse transcription event. In-vivo studies of recombination generally measure the accumulation of recombinant strains over time, and thus do not directly estimate a comparable template switching rate. METHOD: To examine whether the estimated in-vitro template switching rate is representative of the rate that occurs during HIV infection in vivo, we adopted a novel approach, analysing single genome sequences from early founder viruses to study the in-vivo template switching rate in the env region of HIV. RESULTS: We estimated the in-vivo per cycle template switching rate to be between 0.5 and 1.5/1000 nt, or approximately 5-14 recombination events over the length of the HIV genome. CONCLUSION: The in-vivo estimated template switching rate is close to the in-vitro estimated rate found in primary T lymphocytes but not macrophages, which is consistent with the majority of HIV infection occurring in T lymphocytes.

History

Journal

AIDS : official journal of the international AIDS society

Volume

30

Pagination

185-192

Location

Philadelphia, Pa.

eISSN

1473-5571

Language

eng

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2016, Wolters Kluwer Health

Issue

2

Publisher

Lippincott Williams & Wilkins

Usage metrics

    Research Publications

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC