File(s) under permanent embargo
Estrogen-related receptor γ is an in vivo receptor of bisphenol A
journal contribution
posted on 2014-07-01, 00:00 authored by M Tohmé, S Prud’homme, A Boulahtouf, E Samarut, F Brunet, L Bernard, W Bourguet, Y Gibert, P Balaguer, V LaudetBisphenol A (BPA) is an endocrine disruptor that displays estrogenic activity. Several reports suggest that BPA may have estrogen receptor-independent effects. In zebrafish, 50 μM BPA exposure induces otic vesicle abnormalities, including otolith aggregation. The purpose of this study was to test if BPA action was mediated in vivo during zebrafish development by the orphan nuclear estrogen related receptor (ERR) γ. Combining pharmacological and functional approaches, we demonstrate that the zebrafish ERRγ mediates BPA-induced malformations in otoliths. Using different bisphenol derivatives, we show that different compounds can induce a similar otolith phenotype than BPA and that the binding affinity of these derivatives to the zebrafish ERRγ correlates with their ability to induce otolith malformations. Morpholino knockdown of ERRγ function suppresses the BPA effect on otoliths whereas overexpression of ERRγ led to a BPA-like otolith phenotype. Moreover, a subphenotypical dose of BPA (1 μM) combined with ERRγ overexpression led to a full-dose (50 μM) BPA otolith phenotype. We therefore conclude that ERRγ mediates the otic vesicle phenotype generated by BPA. Our results suggest that the range of pathways perturbed by this compound and its potential harmful effect are larger than expected.—Tohmé, M., Prud’homme, S. M., Boulahtouf, A., Samarut, E., Brunet, F., Bernard, L., Bourguet, W., Gibert, Y., Balaguer, P., Laudet, V. Estrogen-related receptor γ is an in vivo receptor of bisphenol A.
History
Journal
FASEB journalVolume
28Issue
7Pagination
3124 - 3133Publisher
Federation of American Societies for Experimental BiologyLocation
Bethesda, Md.Publisher DOI
ISSN
0892-6638Language
engPublication classification
C1 Refereed article in a scholarly journalUsage metrics
Keywords
Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiologyCell BiologyLife Sciences & Biomedicine - Other Topicsendocrine disrupterszebrafishotolithZEBRAFISH DANIO-RERIOENVIRONMENTAL ENDOCRINE DISRUPTORSGENE-EXPRESSION ANALYSISERR-GAMMASMALL EXPOSURESCDNA MICROARRAYCELL-LINECHEMICALSACTIVATIONMECHANISMSPhysiology