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Ethanol as an alternative to formaldehyde for the enhancement of manganese(IV) chemiluminescence detection.
journal contribution
posted on 2014-12-01, 00:00 authored by Zoe SmithZoe Smith, Jessica Terry, Neil BarnettNeil Barnett, Paul FrancisPaul FrancisPrevious applications of manganese(IV) as a chemiluminescence reagent have required the use of formaldehyde to enhance the emission intensity to analytically useful levels. However, this known human carcinogen (by inhalation) is not ideal for routine application. A wide range of alternative enhancers have been examined but to date none have been found to provide the dramatic increase in chemiluminescence intensities obtained using formaldehyde. Herein, we demonstrate that ethanol offers a simple, safe and inexpensive alternative to the use of formaldehyde for manganese(IV) chemiluminescence detection, without compromising signal intensity or sensitivity. For example, chemiluminescence signals for opiate alkaloids using 50-100% ethanol were 0.8-1.6-fold those using 2M formaldehyde. This innocuous alternative enhancer is shown to be a particularly effective for the direct detection of thiols and disulfides by manganese(IV) chemiluminescence, which we have applied to a simple HPLC procedure to determine a series of biomarkers of oxidative stress.
History
Journal
TalantaVolume
130Pagination
221 - 225Publisher
ElsevierLocation
Amsterdam , NetherlandsPublisher DOI
eISSN
1873-3573Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2014, ElsevierUsage metrics
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