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Evaluation of cell disruption method for lipase extraction from novel thraustochytrids

journal contribution
posted on 2017-07-01, 00:00 authored by Avinesh Byreddy, N M Rao, Colin BarrowColin Barrow, Munish PuriMunish Puri
Marine microorganisms represent a rich source of enzymes with the potential industrial application. In this study, we screened novel thraustochytrids for a range of enzymatic activities. The similarity between isolates based on enzyme activities was investigated using cluster analysis. Thraustochytrids were observed to possess different enzymatic activities, however, we focused on lipase activity. Two strains AMCQ-4b27 (34 IU/g) and AMCQS1-9 (36 IU/g) exhibited highest intracellular lipase activity among the isolates. To extract lipase, four different cell disruption methods such as bead vortexing, grinding, sonication and homogenization, were evaluated for their efficiency on lipase extraction yields and quality. Sonication was found to be the best method for enhancing lipase extraction yields from Thraustochytrium sp. AMCQ-4b27 (0.903 IU/μg) and Schizochytrium AMCQS1-9 (1.44 IU/μg).

History

Journal

Algal research

Volume

25

Pagination

62 - 67

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

2211-9264

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2017, Elsevier