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Evaluation of fatty acid extraction methods for Thraustochytrium sp. ONC-T18
journal contribution
posted on 2007-06-13, 00:00 authored by A Burja, R Armenta, H Radianingtyas, Colin BarrowColin BarrowVarious extraction methods were assessed in their capacity to extract fatty acids from a dried biomass of Thraustochytrium sp. ONC-T18. Direct saponification using KOH in ethanol or in hexane:ethanol was one of the most efficient techniques to extract lipids (697 mg g-1). The highest amount of fatty acids (714 mg g-1) was extracted using a miniaturized Bligh and Dyer extraction technique. The use of ultrasonics to break down cell walls while extracting with solvents (methanol:chloroform) also offered high extraction yields of fatty acids (609 mg g-1). Moreover, when the transesterification mixture used for a direct transesterification method was doubled, the extraction of fatty acids increased approximately 77% (from 392 to 696 mg g-1). This work showed that Thraustochytrium sp. ONC-T18 has the ability to produce over 700 mg g-1 of lipids, including more than 165 mg g-1 of docosahexaenoic acid, which makes this microorganism a potential candidate for the commercial production of polyunsaturated fatty acids. Finally, other lipids, such as myristic, palmitic, palmitoleic, and oleic acids, were also produced and recovered in significant amounts (54, 196, 123, and 81 mg g-1), respectively.
History
Journal
Journal of agricultural and food chemistryVolume
55Issue
12Pagination
4795 - 4801Publisher
American Chemical SocietyLocation
Columbus, OhioPublisher DOI
ISSN
0021-8561eISSN
1520-5118Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2007, SpringerUsage metrics
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