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Evaluation of fatty acid extraction methods for Thraustochytrium sp. ONC-T18

journal contribution
posted on 2007-06-13, 00:00 authored by A Burja, R Armenta, H Radianingtyas, Colin BarrowColin Barrow
Various extraction methods were assessed in their capacity to extract fatty acids from a dried biomass of Thraustochytrium sp. ONC-T18. Direct saponification using KOH in ethanol or in hexane:ethanol was one of the most efficient techniques to extract lipids (697 mg g-1). The highest amount of fatty acids (714 mg g-1) was extracted using a miniaturized Bligh and Dyer extraction technique. The use of ultrasonics to break down cell walls while extracting with solvents (methanol:chloroform) also offered high extraction yields of fatty acids (609 mg g-1). Moreover, when the transesterification mixture used for a direct transesterification method was doubled, the extraction of fatty acids increased approximately 77% (from 392 to 696 mg g-1). This work showed that Thraustochytrium sp. ONC-T18 has the ability to produce over 700 mg g-1 of lipids, including more than 165 mg g-1 of docosahexaenoic acid, which makes this microorganism a potential candidate for the commercial production of polyunsaturated fatty acids. Finally, other lipids, such as myristic, palmitic, palmitoleic, and oleic acids, were also produced and recovered in significant amounts (54, 196, 123, and 81 mg g-1), respectively.

History

Journal

Journal of agricultural and food chemistry

Volume

55

Issue

12

Pagination

4795 - 4801

Publisher

American Chemical Society

Location

Columbus, Ohio

ISSN

0021-8561

eISSN

1520-5118

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2007, Springer