Deakin University
Browse
mcgee-exerciseandglut4-2018.pdf (902.88 kB)

Exercise and GLUT4 in human subcutaneous adipose tissue

Download (902.88 kB)
Version 3 2024-06-18, 11:40
Version 2 2024-06-03, 10:12
Version 1 2018-11-27, 10:31
journal contribution
posted on 2024-06-18, 11:40 authored by M Flores-Opazo, E Boland, Andrew GarnhamAndrew Garnham, RM Murphy, Sean McgeeSean Mcgee, M Hargreaves
To examine the effect of acute and chronic exercise on adipose tissue GLUT4 expression, a total of 20 healthy, male subjects performed one of two studies. Ten subjects performed cycle ergometer exercise for 60 min at 73 ± 2% VO2 peak and abdominal adipose tissue samples were obtained immediately before and after exercise and after 3 h of recovery. Another 10 subjects completed 10 days of exercise training, comprising a combination of six sessions of 60 min at 75% VO2 peak and four sessions of 6 × 5 min at 90% VO2 peak, separated by 3 min at 40% VO2 peak. Abdominal adipose tissue and vastus lateralis muscle samples were obtained before training and 24 h after the last training session. A single bout of exercise did not change adipose tissue GLUT4 mRNA; however, there was a small, but significant, reduction in adipose tissue GLUT4 protein expression 3 h after exercise. There were no changes in adipose tissue GLUT4 or COX-IV expression following exercise training. In contrast, skeletal muscle GLUT4 and COX-IV were increased by 47% and 44%, respectively following exercise training. The exercise training-induced increase in GLUT4 expression was similar in both type I and type IIa single muscle fibers. Our results indicate that neither a single exercise bout, nor 10 days of exercise training, increased adipose tissue GLUT4, in contrast with the increases observed in skeletal muscle GLUT4 expression.

History

Journal

Physiological Reports

Volume

6

Article number

ARTN e13918

Pagination

1 - 8

Location

United States

ISSN

2051-817X

eISSN

2051-817X

Language

English

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2018, The Authors

Issue

22

Publisher

WILEY