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Exploring the free radical trapping ability of green tea extracts by optimizing extraction conditions

Version 2 2024-06-04, 15:23
Version 1 2023-10-23, 02:32
journal contribution
posted on 2024-06-04, 15:23 authored by F Ashfaq, MS Butt, A Bilal, Hafiz SuleriaHafiz Suleria
Background: The importance of polyphenols to curb lifestyle related disorders has also raised the worth of green tea polycatechins. Besides, optimization of extraction conditions is gaining wide acceptance to attain maximum polyphenols out of plant matrix. Methods: Considering the fact, in the present study, nine green tea solvent extracts were prepared by optimizing solvents; aqueous ethanol, aqueous acetone and aqueous ethyl acetate in 1:1 ratio and extraction time; 35, 50 & 65 min at constant temperature 50ºC to achieve an optimal quantity of green tea polycatechins. Results: The results indicated significant impact of extraction solvents and time on phytochemistry and free radical scavenging potential with maximum potential attained by acetone extracts of green tea at 50 min i.e. total polyphenols 1325.81±64.85 mg GAE/100g, flavonoids 139.78±5.42 mg/100g, DPPH (1,1- diphenyl-2-picrylhydrazyl) inhibition 86.69±4.01%, β-carotene & linoleic acid assay 78.47±3.57%, FRAP (Ferric Reducing Antioxidant Power) value 19.36±0.94 mM/L Fe+2, ABTS [2, 2'-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid)] value 11.10±0.49 μM Trolox Eq/g and metal chelating activity 16.93±0.81 mg EDTA/g. Conclusion: Conclusively, acetone as solvent and 50 min extraction time have proven optimized extraction conditions for green tea polycatechins.

History

Journal

Current Bioactive Compounds

Volume

15

Pagination

540-546

Location

Beijing, China

ISSN

1573-4072

eISSN

1875-6646

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

5

Publisher

Bentham Science Publishers Ltd.

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