craig-exploringtheutility-2013.pdf (715.46 kB)
Exploring the utility of human DNA methylation arrays for profiling mouse genomic DNA
journal contribution
posted on 2013-07-01, 00:00 authored by Nicholas C Wong, Jane Ng, Nathan E Hall, Sebastian Lunke, Marika Salmanidis, Gabriela Brumatti, Paul G Ekert, Jeffrey CraigJeffrey Craig, Richard SafferyIllumina Infinium Human Methylation (HM) BeadChips are widely used for measuring genome-scale DNA methylation, particularly in relation to epigenome-wide association studies (EWAS) studies. The methylation profile of human samples can be assessed accurately and reproducibly using the HM27 BeadChip (27,578 CpG sites) or its successor, the HM450 BeadChip (482,421 CpG sites). To date no mouse equivalent has been developed, greatly hindering the application of this methodology to the wide range of valuable murine models of disease and development currently in existence. We found 1308 and 13,715 probes from HM27 and HM450 BeadChip respectively, uniquely matched the bisulfite converted reference mouse genome (mm9). We demonstrate reproducible measurements of DNA methylation at these probes in a range of mouse tissue samples and in a murine cell line model of acute myeloid leukaemia. In the absence of a mouse counterpart, the Infinium Human Methylation BeadChip arrays have utility for methylation profiling in non-human species.
History
Journal
GenomicsVolume
102Issue
1Pagination
38 - 46Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
Link to full text
eISSN
1089-8646Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2013, Elsevier Inc.Usage metrics
Keywords
AnimalsCpG IslandsDNADNA FingerprintingDNA MethylationGenome, HumanHumansMiceOligonucleotide Array Sequence AnalysisScience & TechnologyLife Sciences & BiomedicineBiotechnology & Applied MicrobiologyGenetics & HeredityInfinium Bead ChipXeno-arrayHumanMouseEpigeneticsGENE-EXPRESSIONALIGNMENTTOOLInformation SystemsGenetics
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