Deakin University
Browse

File(s) not publicly available

Expression of the human Menkes ATPase in Xenopus laevis oocytes

Version 2 2024-06-03, 16:19
Version 1 2017-05-16, 14:53
journal contribution
posted on 2024-06-03, 16:19 authored by KD Bissig, Sharon La FontaineSharon La Fontaine, Julian MercerJulian Mercer, M Solioz
Menkes disease is an X-linked disorder of copper metabolism that is usually fatal. The affected gene has recently been cloned and encodes one of the two human copper ATPases. If the Menkes ATPase is defective, copper is trapped in the intestinal mucosa, leading to systemic copper deficiency. In order to study copper transport by this ATPase and the effects of disease mutations on its function, we developed a Xenopus laevis oocyte expression system. Wild-type Menkes ATPase cDNA and a fusion of this gene with the green fluorescent protein (GFP) gene was transcribed in vitro and the mRNA injected into oocytes. Expression in oocytes was analyzed by Western blotting and fluorescence microscopy. The Menkes ATPase-GFP chimera appeared to localize primarily to the plasma membrane as assessed by confocal microscopy. This system should thus provide an interesting new tool to study the function of the Menkes ATPase.

History

Journal

Biological chemistry

Volume

382

Pagination

711-714

Location

Berlin, Germany

ISSN

1431-6730

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

[2001, De Gruyter]

Issue

4

Publisher

De Gruyter