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Extent of reduction of foot-and-mouth disease virus RNA load in oesophageal-pharyngeal fluid after peak levels may be a critical determinant of virus persistence in infected cattle
journal contribution
posted on 2004-01-01, 00:00 authored by Z Zhang, C Murphy, M Quan, J Knight, Soren AlexandersenSoren AlexandersenTo investigate whether foot-and-mouth disease virus (FMDV) RNA loads in oesophageal-pharyngeal fluid (OP-fluid) in the early course of infection is related to the outcome of virus persistence, viral RNA in OP-fluid samples from cattle experimentally infected with FMDV type O was quantitatively analysed by using a quantitative real-time RT-PCR. Viral RNA was detected within 24 h post-infection (p.i.) in all infected animals. Rapid virus replication led to peak levels of viral RNA load by 30-53 h p.i., and then the load declined at various rates. In some animals (n=12, so-called non-carriers) viral RNA became undetectable between 7 and 18 days p.i. In contrast, in persistently infected animals (n=12, so-called carriers) viral RNA persisted in OP-fluid samples at detectable levels beyond 28 days p.i. Analysis of early viral decay/clearance and virus clearance half-life in OP-fluid samples showed that the extent of reduction of viral RNA in OP-fluid samples immediately following peak levels is a critical determinant of the outcome of FMDV persistence.
History
Journal
Journal of general virologyVolume
85Pagination
415 - 421Publisher
Microbiology SocietyLocation
London, Eng.Publisher DOI
Link to full text
ISSN
0022-1317Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2004, SGMUsage metrics
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AnimalsBody FluidsCarrier StateCattleEsophagusFoot-and-Mouth DiseaseFoot-and-Mouth Disease VirusPharynxRNA, ViralReverse Transcriptase Polymerase Chain ReactionTherapeutic IrrigationViral LoadScience & TechnologyLife Sciences & BiomedicineBiotechnology & Applied MicrobiologyVirologyPOLYMERASE CHAIN-REACTIONRT-PCR ASSAYREAL-TIMECARRIER CATTLECELLSPIGSPATHOGENESISGROWTHSHEEP
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